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KMID : 1059519900340050424
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Journal of the Korean Chemical Society
1990 Volume.34 No. 5 p.424 ~ p.429
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Preparation of the Citrobacter freundii Bio-Sensor for the Determination of Glucose and Its Applications
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Ihn Gwon-Shik
Hong Young-Seuk
Kim Ui-Rak
Jang Seh-Yong
Sohn Moo-Jeong
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Abstract
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A bio-sensor for the determination of glucose has been constructed by immobilizing the Citrobacter freundii or its organelle on carbon dioxide gas-sensor. The bacterial sensor was better than organelle in response, but the latter showed a shorter response time. The bacterial sensor gave linearity between 7.0 × 10-4 and 1.0 × 10-2 M glucose with a slope of 42.2 mV/decade in pH 7.0, 0.2 M tris-HCl buffer at 30℃. The selectivity of this sensor was very high for glucose. Employing for the determination of glucose in serum, the sensor showed a good agreement with a routine analyzer.
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